Early Events in the Infection of Tobacco with Alfalfa Mosaic Virus
نویسندگان
چکیده
منابع مشابه
Infection of tobacco mesophyll protoplasts by tobacco mosaic virus.
It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 10(6) virus particles per infected protoplast during 24 hours of incubation. The...
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The four alfalfa mosaic virus RNAs (respectively 24 S, 20 S, 17 S and 12 S) have been used separately as messengers in two in vitro protein synthesizing systems: wheat germ and rabbit reticulocyte lysate. In both systems a polypeptide corresponding to the translation of the entire length of the RNA can be found for RNAs 24 S, 20 S and 12 S, but not for 17 S RNA, the translation product of which...
متن کاملInfection of tobacco mesophyll protoplasts by cucumber mosaic virus.
Protoplasts isolated from tobacco leaves were inoculated with cucumber mosaic virus (CMV) using a method slightly modified from that developed for inoculating tobacco mosaic virus. Multiplication of CMV was demonstrated by staining the protoplasm with fluorescent CMV-antibody, by the electron microscopy of ultrathin sections, and by assaying the infectivity in protoplasts. Seventy to 90% of pro...
متن کاملA simplified method of obtaining tobacco protoplasts for infection with tobacco mosaic virus.
Incubating tobacco leaf tissue, from which the lower epidermis was peeled, overnight with o'3 to o.4 % Macerozyme and o-6 to i-2 % cellulase, depending on leaf condition, produced a good yield of protoplasts that were susceptible to infection by TMV. Fluorescent antibody staining showed that 20 to 8o % ofprotoplasts became infected, and infectivity tests indicated that an average of about 4 × I...
متن کاملThe spreading of Alfalfa mosaic virus in lavandin in Croatia
A survey was conducted in 2012 and 2013 to detect the presence and distribution of Alfalfa mosaic virus (AMV) in lavandin crops growing in continental parts of Croatia. A total of 73 lavandin samples from six crops in different localities were collected and analyzed for the presence of AMV and Cucumber mosaic virus (CMV) using commercial double-antibody sandwich (DAS)-ELISA kits. AMV was detect...
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ژورنال
عنوان ژورنال: Journal of General Virology
سال: 1978
ISSN: 0022-1317,1465-2099
DOI: 10.1099/0022-1317-41-3-649